A field experiment was conducted during the winter (Rabi) season at Bulandshahr, Uttar Pradesh, under Centrally sponsored scheme (NHM) Ministry of Agriculture, GOI, Calicut, Kerala to assess the response of fenugreek (Trigonella foenum-graecum L.) to fertility levels and bio-fertilizers. Three fertility levels [50, 75 and 100% recommended dose of fertilizers (RDF): 40 kg N + 40 kg P₂O₅ + 10 kg S/ha)] in main plots and 3 bio-fertilizer inoculations [Rhizobium, phosphate-solubilizing bacteria (PSB) and Rhizobium + PSB] in subplots were studied in split-plot design with 3 replications. Significantly the highest values of growth parameters, nodules/plant, yield attributes, seed yield (2.25 t/ha) and haulm yield (5.25 t/ha), were recorded with application of 100%RDF over lower fertility levels. Dual inoculation of seed with Rhizobium + PSB resulted higher growth, nodules/plant, yield attributes and seed yield (2.10 t/ha) over their sole application. Interaction of fertility levels and bio-fertilizers was also found significant in yield attributes and in seed yield also. Application of 100% RDF + dual inoculation, being at par with 75% RDF + dual inoculation, gave the highest seed yield (2,287 kg/ha).The integration of 75% RDF with dual inoculation in respect of yield attributes and seed yield were recorded significantly higher.