The aim of this investigation was to find out the effects of some biopesticides (Trichoderma and Neem) at different concentrations on mitotic index and mitotic aberrations on Vicia faba L. plants. The poisonous effects of some chemicals used for agriculture practices and responsible for increasing the environmental pollution which is a recent worldwide problem. So that several experiment were taken to find out poisonous activity of chemicals /natural compounds for more ecological safety. Effect of different concentration of biopesticides that is 150ppm, 300ppm, 550ppm and 800ppm for 8 hrs has been observed. The mitotic index decreases due exposure to plant extract in higher concentration and longer duration period. The mitotic index decreases due exposure to Trichoderma solution in higher concentration and longer duration period. The mitotic index is minimum at 800 ppm concentration of Trichoderma (solution) for 8 hrs that is 17.81 which is lower from control that is 22.11.The anti mitotic effect was in low frequency at 300 ppm of for,8 hrs was 21.89,21.06, 20.95 respectively. While neem was less effective than Trichoderma by showing less reduction in MI that is at 800 ppm MI is 20.6 which is lower than MI in control.