The present investigation was carried out in pot experiment. Collection of rhizosphere and non rhizosphere soil was done at 41 and 111 days of sowing. In the present study association of mycoflora in rhizosphere under the influence of different manures was investigated in spinach plant. A total of 14 fungal species belonging to different groups viz., Aspergillus niger, A. flavus, A. terreus, A. roseus, Trichoderma viride, Penicillium sp., Colletotrichum spinacease, Rhizoctonia solani, Fusarium oxysporum, Alternaria sp.,Rhizopus mucar, Penicillium, Chaetomium sp., Cladosporium sp. were isolated. Among them Aspergillus was the most predominant genus. Analysis of soil samples from all treated as well as control plants showed that the saprophytic fungi were more frequent than pathogenic fungi due to effect of weed green manures.