The experiment was con ducted at the Department of Horticulture, Kulbhasker Ashram Post Graduate College, Allahabad, Uttar Pradesh with a view to standardize suitable stratification duration and hormone concentration for guava seed treatment. There were seven treatment combinations including a control. Different duration of seed stratification i.e., 24 hours, 48 hours and 72 hours were tried along with the 100 ppm, 200 ppm and 300 ppm GA₃ seed treatment. Treated seeds were sown in the polythene bags (25 x15 cm size, 200 gauge thick) containing soil, sand and FYM mix ture (1:1:1). It was interesting to note that the effect of stratification duration and hormone treatment concentration was found to be significant for seed germination, transplanting success, seedling mortality percentage and rate of seed germination. Treatment T6 (48 hrs stratification + 300 ppm GA₃ ) resulted in highest percentage (51.0 %) of seed germination while the lowest percentage (23.25) was recorded in T₉ (72 hrs + 300 ppm GA3) treatment and the transplanting success was also lowest in T₉. The seedling mortality percentage was maximum (62.66%) with T₉ whereas lowest percentage (17.0%) was observed in T₆ treatment. It may be concluded that T₆ treatment can be recommended for the better stand establishment of guava nursery.