The present study on genetic variability, heritability and genetic advance in tomato germplasm for horticultural traits under Lucknow conditions was carried out at the Horticulture Research Farm of Department of Applied Plant Science, BBA University, Vidya Vihar, Rae Bareli Road, Lucknow (U.P.), India, during Rabi season of 2015-16. On the basis of overall findings, it was concluded that there is wide range of variation in tomato strains for all the characters studied. The strain PT-11 is highly significant for days to marketable picking, number of flowers/cluster, number of fruits/cluster, number of fruit/plant, fruit weight/ cluster and fruit yield, and the biochemical traits viz., TSS and ascorbic acid were found highest in PH-2 strain of tomato except acidity.