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  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
Journal : HortFlora Research Spectrum
Volume : Volume 6, Issue 2: June 2017
Page (s) : 93-96
Date of Publication
Print :
Article Type : Full Length Original Article
Online : 02-Jul-2017
Optimization of Protocol for Minimizing in-vitro Contamination of Embryo Cultured Peach (Prunus persica L.)
A.S. Sundouri*, Harminder Singh , Anirudh Thakur and Dimpy Raina
*Corresponding Author's E-mail :


Micro propagation is a rapid propagation technique for raising any kind of plant but it encounter with the utmost problem of contaminations by different sources such as bacteria, fungi and many more which reduces their productivity and completely prevents their successful cultivation. The present study was an attempt to minimize the contamination occurred in the embryo cultured peach by using different surface sterilants to obtain successful plantlet. Among the different sterilants cetrimide 5% for 15 minutes followed by carbandazium 2% for 5 minutes was the most effective treatment which showed maximum effect against contaminations. However, the sterilant 0.1% HgCl2 for 10 minutes and ethanol for 70% for 3 minutes duration gave maximum uncontaminated plantlet with low survival rate.

Cite this article as:
Sundouri A.S., Singh H., Thakur A. and Raina D. (2017). Optimization of protocol for minimizing in-vitro contamination of embryo cultured peach (Prunus persica L.). HortFlora Res. Spectrum, 6(2) : 92-96.
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