Tuberose (Polianthes tuberosa) is one of the popular flower crop cultivated for loose flower and cut flower in India and also holds a great potential for export. Therefore, an experiment was conducted at the research farm of ICAR-Directorate of Floricultural Research, Shivajinagar, Pune to evaluate performance of seven genotypes of tuberose viz., Mexican Single, Sikkim Selection, Arka Shringar, Phule Rajani, Hyderabad Single, Arka Prajwal and Arka Nirantara for their performance under Pune condition. The study revealed that significant variation was recorded on vegetative growth and floral parameters among the evaluated genotypes. Cultivar Arka Prajwal produced significantly taller plants (62.27 cm) followed by Arka Nirantara (60.27 cm). Cultivar Phule Rajani (23.86) recorded highest number of bulbs produced per clump followed by Hyderabad Single (18.00) and Mexican Single (11.60). Genotype Sikkim Selection produced tallest spike (108.43 cm) followed by cv. Arka Prajwal (104.41 cm). Cultivar Arka Prajwal produced significantly higher rachis length (34.70 cm), fresh weight of cut spike (121.67 g), diameter of open florets (4.36 cm) and weight of 20 open (37.00 g) and mature (27.33 g) floret compared to other genotypes. Cultivar Arka Nirantara produced maximum number of florets per spike (57.60) followed by cv. Arka Prajwal (53.33).