A field trial was conducted at Horticulture Research Centre, Chauras Campus H.N.B University,arhwal, Uttarakhand, during October 2014 to February 2015 to study the growth and yield potential of broccoli influenced by combined effect of organic manures and bio-fertilizer under Garhwal Himalayan region. The experiment was laid out in randomized block design with three replications. The experiment consist of two organic manures and bio fertilizer with their combinations viz., Vermicompost (T₁), Poultry manures (T₂), Azotobacter (T₃), Vermicompost + Poultry manure (T₄), Vermicompost + Azotobacter (T₅), Poultry manure + Azotobacter (T₆), Vermicompost + Poultry manure + Azotobacter (T7), Control (T8). All the organic manures were applied at the field before one month of transplanting but Azotobacter were applied as root treatment before transplanting. Results were found to be significant in all the growth and yield parameters of broccoli. T₇ (Vermicompost + Poultry manure + Azotobacter) showed maximum plant height (57.13 cm), leaf length (48.17 cm), plant spread (56.16 cm²), days to curd initiation (65.89), days to 50% curd initiation (75.23) and days taken to 50% curd maturity (88.51), where as maximum number of leaves (22.58), curd weight (315.69 g) and yield/ha (120.12 q) recorded in T₆ (Poultry manure + Azotobacter), while minimum under T₈ (Control). The results showed that the combined use of organic and bio-fertilizer significantly effective on growth and yield characters.