In India, oyster mushrooms are particularly interesting as one kind of popular foods. The present study was conducted with the aim of finding out the most favourable temperature, pH and different media on radial growth rate were assessed on potato dextrose agar medium (PDA). Study was carried out to check the effect of temperature (23 - 28°C), pH (6.9 - 8.1) and different media (Potato dextrose agar, Chickpea extract agar, Pigeon pea extract agar, Barley extract, Black gram extract agar, and Oat extract agar) on the radial growth of Pleurotus djamor. Optimum temperature and pH for growth was 28°C and 7.5, respectively. Maximum radial growth was observed when Barley extract agar was used as media.