The experiment was pursued in Tissue Culture Laboratory of Department of Horticulture in Sardar Vallabhbhai Patel University of Agriculture & Technology Meerut during 2015-16 on Chandler variety of strawberry. N₆ media were prepared. Maximum callus formation in mature leaf explants (81%) was noted under the treatment of BAP 2mgl^-1 combined with IBA 1.0 mgl^-1. Maximum callus formation in young leaf (74.0%) was noted under the treatment of BAP 2mgl^-1 combined with IBA 1.0mgl^-1.Maximum callus induction in internodes (47.6%) was noted under the treatment of BAP 2mgl-1 combined with IBA 1.0 mgl^-1 . Highest number of shoots (14.00) from mature derived callus at four weeks after inoculation were noted under the treatment of BAP 2mgl-1+ Kinetin 1.5 mgl^-1. Maximum number of shoots (11.66) from young leaf derived callus of strawberry cv. Chandler at four weeks after inoculation were noted under BAP 2mgl^-1 combined with Kinetin 1.5 mgl^-1 and BAP 3mgl^-1 alone. The highest number of shoots (10.33) from internodes derived callus at four weeks after inoculation were noted with BAP 3mgl^-1 alone. Viewing above observations it is concluded that BAP 2mgl^-1 + IBA 1.5 mgl^-1 and Kinetin 1.5mgl^-1 + IBA 1.0mgl^-1 showed better performance on accordance of callus formation in mature leaf, young leaf as well as internodes. BAP 2mgl^-1 + Kinetin 2mgl^-1 showed better performance on accordance of shoot induction in mature leaf, young leaf as well as internodes.