The present experiment was conducted to study the effect of bio and chemical fertilizers on plant vigour, flowering and yield of petunia (Petunia hybrida) var. Picotee in the Department of Horticulture, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, (U.P.) during the winter season 2014-2015. The results revealed that treatments T₁₃ (Azotobactor + PSB +PMB + 100% doses of NPK) had significant response most of the traits studied. The maximum plant height (29.80 cm), number of leaves/plant (600.60) number of branches/plant (20.00), plant spread (54.30 cm), first flower bud emergence (53.06, diameter of flower/(8.26 cm), number of flowers/plant (76.93), weight of fresh flower (1.05g) and weight of dry flower (0.81g) were produced by the treatment T₁₃ (Azotobactor + PSB +PMB + 100% doses of NPK).