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  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
Journal : HortFlora Research Spectrum
Volume : Volume 2, Issue 4: October-December 2013
Page (s) : 319-323
Date of Publication
Print :
Article Type : Full Length Original Article
Online : 24-Dec-2013
Effective Inoculation Method and Optimum Concentration of Oryctes Virus for Biological Control of Coconut Beetle (Oryctes rhinoceros) Adults
S. P. Jayawardena*
*Corresponding Author's E-mail :


The study was carried out to determine the effective inoculation method and optimum concentration of local Oryctes virus (OrV) for successful infection of Oryctes rhinoceros (L.) adults in laboratory which released to the field to spread the viral disease among healthy larvae and beetles for their biological control. 0.1ml of 104 ppm of viral suspension was introduced orally to one set of beetles and another set of beetles were allowed to swimming in the suspension for 10 minutes. Beetles were dissected at different intervals to determine the period taken for infection. Five concentrations (101 ppm to 105 ppm) of virus suspensions were introduced to adult beetles orally to find the lethal concentration (LC50) and lethal time (LT50). Percentage of infected beetles was significantly different (P<0.001) among the two methods of inoculation and untreated control. After 21 days of inoculation, oral introduction method, swim method and control recorded 88.8%, 44.4% and 11.1% of OrV infection, respectively indicating the most effective inoculation method as oral introduction. Cumulative per cent mortality of O. rhinoceros adults with 101 ppm,102 ppm, 103 ppm, 104 ppm and 105 ppm concentrations were recorded as 7.3%, 25.1%, 33.3%, 81.4% and 100%, respectively. LC50 was 102.7 ppm and LT50 for 104 ppm and 105ppm concentrations were 23 days and 12 days, respectively. Best concentration to oral inoculation of O. rhinoceros with OrV for field release was selected as 104 ppm.
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Cite this article as:
Jayawardena S. P. (2013). Effective inoculation method and optimum concentration of Oryctes virus for biological control of coconut beetle (Oryctes rhinoceros) adults. HortFlora Res. Spectrum, 2(4): 319-323
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