Two fistulated male buffalo calves of 2 years age having 277 kg average body weight were selected. Dried and pulverized samples of unconventional feed viz. Banana leaf (A) and stem (B) and Water Hyacinth plant (C) along with Wheat Bhusa were taken in the nylon bags in the ratio of 00:100, 10:90,20:80 and 40:60 and designated as group I, II, III and IV. One type of feed was transferred in the rumen of one animal at a time. The experiments were rotated once in all the animals.The rumen pH was highest at 0h and gradually declined at post feeding hours. Bacterial count increased significantly (P< 0.01) at 4h of feeding and then it is decreased. The digestibility of all the nutrients increased (P< 0.01) at 24 h than the sample obtained at 12 h of collection in all the feeds. The digestibility of feed nutrients was higher when 10% plant samples were mixed with wheat bhusa than rest of the groups. The DM digestibility in feed B and C was significantly higher (P<0.05) in all the groups than in group A.