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  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
  • HortFlora Research Spectrum
Journal : HortFlora Research Spectrum
Volume : Volume 7, Issue 1: March 2018
Page (s) : 58-61
Date of Publication
Print :
31-Mar-2018
Article Type : Full Length Original Article
Online : 31-Mar-2018
Title:
Estimation of Colchicine in Cell Suspension Cultures of Gloriosa superba L.
Author(s):
Pallavi Billowria, Nisha Kapoor and Ritu Mahajan*
*Corresponding Author's E-mail :
ritufeb@gmail.com

Abstract

Callus was induced from different explants of Gloriosa superba L. on MS medium supplemented with different concentrations of 2,4- D. Cell suspension cultures derived from callus of Gloriosa superba L. were established quantified for the colchicine content using High Performance Liquid Chromatography. Effect of time on the growth of cells and colchicine production by cell suspension cultures in liquid medium was also studied. Maximum growth index (0.331 ± 0.0016) was obtained after four weeks of cell growth. Itwas observed that the colchicine yield reached 13.33 ± 0.024 μg/g dry wt. in cells and 59.30 ± 0.54 μg/l in residual liquid medium after four weeks of culture in liquid growth medium and thereafter there was reduction in biomass as well as colchicine content.
 

Cite this article as:
Billowria P., Kapoor N. and Mahajan R. (2018). Estimation of colchicine in cell suspension cultures of Gloriosa superba L. HortFlora Res. Spectrum, 7(1) : 58-61.
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