Mulberry belongs to the genus Morus and family Moraceae. Mulberry is distributed in tropical, subtropical and temperate zones. Conventional breeding is limited in mulberry due to high heterozygosity and long generation period. Many of the newly developed mulberry varieties cannot be propagated through stem cutting. Many desired cultivars do not root easily or have low rooting ability. Such difficult varieties could be multiplied by using tissue culture techniques. Micro propagation is a technique used to produce the plants in vitro by providing requirements for their growth, using proper growth hormones, through which plants can multiply and regenerates in in vitro conditions and as a result we can multiply plants using suitable explants on nutrient media. Advanced methodologies in micro propagation of mulberry have been made by several researchers using different explants and media. The present review is pertaining to the in vitro studies which have already been carried out in Morus.