To investigate the growth and yield of Aloe (Aloe barbadensis M.) in open condition as influenced by harvest dates, an experiment was carried out at the Medicinal and Aromatic Plants (MAP) Block, Experimental Farm, Department of Horticulture, Assam Agricultural University in Jorhat during 2015-2016. A total of four treatments with five replications were laid out in a Randomized Block Design. The treatments were comprised of: T₀- Harvesting at an interval of two months (recommended practice), T₁- Harvesting at one and a half months interval (after first harvest), T₂- Harvesting at two and a half months interval (after first harvest) and T₃- Harvesting at three and a half months interval (after first harvest). The results showed that the different harvest intervals had significant effect on the growth and yield of Aloe. The maximum values for all the growth and yield parameters were observed in T₃ [Harvesting at three and a half months interval (after first harvest)] treatment whereas the minimum values were observed in T₁ [Harvesting at one and a half months interval (after first harvest)] treatment. Over all, Aloe leaves harvested at three and a half months interval resulted in higher productivity and maximum benefit in subtropical climate of Jorhat, Assam.